LabPAGE 4-12� 15 Wells

La description

LabPAGE 4-12％ 15 Wells （CAT：P41215）

• Price: U.S.＄48.00 for 10 pieces

  Gel Separation Range

  LabPAGE™ Precast Protein Gel is available in fixed concentration precast and gradient precast.Fixed concentration Precast Protein Gel has three concentrations: 8%,10% and 12%,and gradient concentration Precast Protein Gel has two concentrations: 4%-12% and 4%-20%.

  

   

  Note: When using this product,please be sure to use a special electrophoresis buffer,it is recommended to directly use the company's matching special electrophoresis solution: MOPS-SDS Running Buffer (Cat.No:T7205M，10*1 L）。The recommended number of running buffer reuses is no more than 3 times.

  Instructions

  LabPAGE™ Precast Protein Gel are polyacryamide gels designed for Proteins Separation.A gel has 12 wells or 15 wells formats .For gel with 12 wells its largest volume is up to 50 uL per well,recommended loading volume of 25 μL or less;while for gel with 15 wells,its largest volume is up to 30 uL per well,recommended loading volume of 15 μL or less.

  LabPAGE™ Precast Protein Gel adopts automatic control technique for gel perfusion which enables good repeatability and stability .Its unique formulation makes bands more clear and sharp which increases the evenness of the band and provides superior resolution.

  They run at neutral pH buffer which not only minimizes protein modification but also improves gel stability.

   

  Product Benefits

  (1)Compared with the traditional laboratory self-preparation gel

  open-Use:There is no need to prepare a variety of solutions and glue operation,ready to use.

  Time-Saving:The process of electrophoresis can be finished in just 30minutes greatly saving our precious time

   

   

  Safety-Ensuring:No need to contact toxic reagent

  Stable-Results:Full automatic andlarge-scale production enables good repeatability and high quality.

   

  (2)  Compared to other brands of Precast Protein Gel

  Perfect bands:Unique design, greatly reduce the edge effect and make electrophoresis results more perfect.

  Cost-effective:High-quality domestic goods, with super quality and competitive price.

  High resolution: The unique gel buffer formula makes the protein electrophoresis bands clearer and sharper with higher resolution.

  best match: Each box of Precast Protein Gel comes with two packs of Tris-MOPS-SDS instant granules.

   

  Instructions For Use

  â‘ Buffer preparation: Take out a packet of MOPS-SDS Running Buffer and dissolve it in 1L of deionized water;

  â‘¡Take the precast protein gels out of the package and remove the pink tape at the bottom of the gel plate (see Figure 1);

  â‘¢Push the comb smoothly out of the gel plate according to the direction of the arrow (see Figure 2);

   

  

   

  â‘£Avoid residual liquid when remove the comb;

  ⑤Precautions for using the raised electrophoresis tank (see Figure 3) of the Bio-Rad,WIX,etc.Remove the green silicone seal of the frame inside the tank,then insert it back into the groove of the inner frame with its flat side facing outward.(see Figure 3);

   

  

   

  â‘¥Insert the gel into the gel running apparatus.(See Figure 4 and Figure 5);

   

  

   

  ⑦Pour sufficient MOPS-SDS Running Buffer into the inner tank of the gel running apparatus to cover the sample wells by 5-7 mm, Fill the outer tank with the same running buffer to ensure proper cooling.For best results, the level of the buffer in the outer tank should be below the top level of the buffer in the inner tank, and the buffer can not overflow the cassette; Use a syringe or other tools to draw moderate running buffer to rinse the sample wells and remove air bubbles and displace any storage buffer. Load protein sample and begin the process of electrophoresis.Our recommended voltage is 160 V and the maximum voltage must be no more than 180 V.

  Notes: Please don not use Tris-Glycine electrophoretic buffer solution,because it is not compatible with the the buffer system of the LabPAGEE™ Precast Protein Gel

   

  

   

  ⑧Remove the gel from the plate (See Figure 6).

  a、remove the gel plate from the apparatus at the end of the process of electrophoresis;

  b、Carefully Insert the appropriate warping tool into the gap between the plate;

  c、Pry the upside part,downside and middle part of the cassette until the complete separation of the both sides;

  d、Upon opening,gel may sit on either side of the plate,dip the plate with gel into the water under the surface,slant the plate and then gently lift it.when the gel fall into the water,remove the gel from the water to continue following experiments.