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Localité:
China
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Groupe de produits :
Personne àcontacter Ying
DH5a competent cells
CAT: DH***0 Price :Â
U.S.＄*8.*0 for
**0ul;Â Â $*3for 5***0ul;Â $**5 for *0***0ul
(negotiable)
Storage conditions: **5 ~ **5℃ storage, dry ice
transportation.
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Product description:
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DH5α strain is the most commonly used competent cell in the
laboratory. The lack of endonuclease (endA) improves the yield and
quality of plasmid DNA; the recombinase defect (recA) reduces the
probability of homologous recombination of the inserted fragment
and ensures the stability of the inserted DNA; the presence of
lacZΔM*5 makes DH5α suitable for blue-white screening. Our
company's DH5α competent cells are made by special process, and
the transformation efficiency is ≥**8cfu/μg using pUC*9 plasmid DNA
detection.
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Genotype
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F- φ*0 lac ZΔM*5 Δ(lacZYA-arg F) U**9 endA1 recA1 hsdR*7(rk-,mk+)
supE*4λ- thi *1 gyrA*6 relA1 phoA
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Experimental process
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1. Take out the competent cells from **0℃ and quickly put them on
ice to melt.
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2. Add the DNA to be transformed to **0 μl competent cells, flick
the tube wall to mix (avoid using a gun to suck), and place on ice
for *0 min.
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3. After heat shock in a *2℃ water bath for *5 sec, quickly place
on ice for 2 min. Do not shake the centrifuge tube.
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4. Add **0 μl LB or SOC liquid culture medium (without antibiotics)
to the centrifuge tube, mix well, and place in a *7℃, **0 rpm
shaker for 1 h.
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5. Centrifuge at 5,**0 rpm (2,**0 × g) for 3 min, discard **0 μl
supernatant, resuspend the bacteria with the remaining culture
medium, and evenly spread on the LB solid culture medium plate
containing the corresponding antibiotics.
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6. Place the plate upright in a *7℃ incubator for *0 min. After
the bacterial solution is completely absorbed, invert the plate and
culture overnight.
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Notes:
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1. After thawing in an ice-water bath, the competent cells should
be used immediately. Leaving them for a long time will reduce the
transformation efficiency.
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2. The volume of DNA to be transformed should not exceed 1/*0 of
the volume of competent cells.
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3. After adding plasmid or ligation product, do not use a pipette
to aspirate, just flick to mix.
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4. Avoid repeated freezing and thawing of competent cells.
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| Pays: | China |
| N ° de modèle: | - |
| Prix FOB: | ( Negotiable ) Obtenir le dernier prix |
| Localité: | China |
| Prix de commande minimale: | - |
| Commande minimale: | - |
| Packaging Detail: | - |
| Heure de livraison: | - |
| Capacité de Fournir: | - |
| Payment Type: | T/T, L/C, Western Union, Money Gram, PayPal, Other |
| Groupe de produits : | Molecular Biology |
