Prix FOB
Obtenir le dernier prix20 ~ 20 / Milliliter ( Negotiable )
|20 Milliliter Minimum Order
Pays:
China
N ° de modèle:
-
Prix FOB:
20 ~ 20 / Milliliter ( Negotiable ) Obtenir le dernier prix
Localité:
China
Prix de commande minimale:
20 per Milliliter
Commande minimale:
20 Milliliter
Packaging Detail:
-
Heure de livraison:
7-15 days if in stock
Capacité de Fournir:
1000 Milliliter per Month
Payment Type:
T/T
Groupe de produits :
China
Personne àcontacter Ying
Storage temperature: **0°C for 2 years. For short-term use (within
1 month), place at 4°C
Product description:
This product contains Lab FastHF DNA Polymerase, dNTPs and
optimized reaction buffer at a concentration of 2×. When using,
just add template, primers, and add water to the final
concentration of Mix to 1×. FastHF DNA Polymerase comes from
genetically engineered pfu, which greatly improves the
amplification speed, fidelity and yield. Lab FastHF PCR Master Mix
is ​​the preferred product for non-long fragment ultra-fidelity
rapid amplification. This product contains red tracking dye, and
can be directly loaded for electrophoresis without adding loading
buffer; it can also be purified for subsequent operations such as
enzyme digestion, ligation, and fluorescent sequencing. The PCR
product has a blunt end and does not need to add an A head. It can
be directly cloned using LabpO TOPO-Blunt blunt end series vectors
(Cat. No. PB***1, PB***2).
Product features:
1. Fast amplification speed: The extension speed can reach
**4kb/min, which is **8 times that of pfu.
2. High amplification yield: The general PCR product amount is
*0%***0% higher than the traditional pfu yield.
3. Excellent fidelity: The fidelity is more than *4 times that of
taq. Generally, a random bacterial sequencing is the correct
mutation-free colony.
4. The amplification length index is consistent with pfu, and it
can completely replace pfu for rapid and high-fidelity
amplification, but it is not recommended for long fragments that
pfu cannot amplify. Using complex genomic DNA as a template is
suitable for amplifying products of no more than about 3kb, and
using simple genomes, plasmids and phage DNA as templates is
suitable for amplifying products of no more than about 6kb.
Notes:
1. Lab FastHF DNA Polymerase has a fast amplification speed. Simple
templates such as plasmids and simple genomes can be amplified at
****0 seconds/kb; complex templates such as human genomes can be
amplified at ****5 seconds/kb.
2. For templates with high GC content, the pre-denaturation and
denaturation temperatures can be increased to *8°C. Lab FastHF DNA
Polymerase has strong heat resistance, and *8°C has no effect on
the activity of Lab FastHF DNA Polymerase.
3. If the amplification template has a high GC content or the
template is complex and the amplification effect is poor, DMSO can
be added to the reaction mixture to a final concentration of 1%*8%,
and the optimal concentration can be found by increasing the
concentration by 1%. Alternatively, betaine can be added to a final
concentration of 1.**1.7 M. Touchdown PCR is used.
| Pays: | China |
| N ° de modèle: | - |
| Prix FOB: | 20 ~ 20 / Milliliter ( Negotiable ) Obtenir le dernier prix |
| Localité: | China |
| Prix de commande minimale: | 20 per Milliliter |
| Commande minimale: | 20 Milliliter |
| Packaging Detail: | - |
| Heure de livraison: | 7-15 days if in stock |
| Capacité de Fournir: | 1000 Milliliter per Month |
| Payment Type: | T/T |
| Groupe de produits : | Molecular Biology |
