M-MLV GIII Reverse Transcriptase (third generation enzyme)

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Molecular Biology

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La description

M-MLV GIII Reverse Transcriptase (third generation enzyme)

Cat. No.: M***4

Price: $*0 for ****0U (negotiable if bulk purchase)

Storage conditions: **0â„ƒ

Product composition

Component specification	Specification
M-MLVGIII Reverse Transcriptase(**0U/ul)	*0ul
5×M-MLV First Strand Buffer	**0ul
0.1M DTT	**0ul

Product Introduction

M-MLV GIII Reverse Transcriptase is a third-generation M-MLV reverse transcriptase obtained through gene modification and recombinant technology. Compared with wild-type M-MLV reverse transcriptase, this enzyme removes RNase H activity and greatly improves thermal stability (optimal reaction temperature is *0°C), thereby greatly improving the specificity and length of the first-chain cDNA synthesis (up to *2 kb), and enhancing tolerance to complex secondary structures of RNA.

Enzyme activity unit definition

At *7°C, with Poly(A)-Oligo(dT) as template/primer, within *0 min, the amount of enzyme required to incorporate 1 nmol of [3H] dTTP is defined as 1 activity unit (U).

Quality Control

Host DNA detection

No host DNA contamination

Endonuclease residue detection

The enzyme solution was incubated with supercoiled plasmid DNA at *7°C for 4 hours, and the plasmid was detected by DNA electrophoresis without any changes.

Exonuclease residue detection

Incubate the enzyme solution with double-stranded DNA substrate at *7â„ƒ for *6h. DNA electrophoresis was used to detect no change in the double-stranded DNA substrate.

reagent	usage amount
primer	X ul
Oligo(dT)*0	final concentration2.5uM
Or random primers	final concentration2.5ng/ul
Or gene-specific primers	final concentration0.*5uM
Template RNAa	0 ng~1ug/0ul
5× M-MLV First Strand Buffer	4ul
0.1 M DTT	1ul
M-MLV GIII Reverse Transcriptase (**0 U/ul)	1ul
dNTP Mix (*0 mM Each)	1ul

Pays:	China
N Â° de modÃ¨le:	-
Prix FOB:	(Negotiable) Obtenir le dernier prix
LocalitÃ©:	-
Prix de commande minimale:	-
Commande minimale:	-
Packaging Detail:	-
Heure de livraison:	-
Capacité de Fournir:	-
Payment Type:	-
Groupe de produits :	Molecular Biology