First-strand cDNA Synthesis Mix

La description

Product name:

First-strand cDNA Synthesis Mix (third generation enzyme, independent primers)
 
Cat. No.: F***2O
 
Price: $**0 for *0 ul***0T(negotiable)
 
Storage conditions: **0°C
 
Product components:

components	specification
RTase III Primer Flexible All-in-One Mix	**0μl
Oligo(dT)*0VN (*0μM)	**0μl
Random hexamers (*0μM)	**0μl
Nuclease-Free Water	2×1 ml

Product Introduction
 
RTase III Primer Flexible All-in-One Mix is ​​a high-efficiency, low-pollution, high-quality, single-strand cDNA synthesis premix. It contains M-MLV GIII Reverse Transcriptase and its reaction buffer, RNase inhibitors, dNTPs and other components required for single-strand cDNA synthesis. You only need to add RNA template, primers and water to start the reaction. It is very convenient to operate. Different types of reverse transcription primers can be used flexibly for different experimental designs to meet diverse experimental needs. Oligo(dT)*0VN or Random hexamers or Gene Specific Primers can be selected according to different experimental scenarios. Using this reverse transcription premix, a maximum of *2 kb cDNA can be obtained within *5 minutes.
 
Instructions
 
Prepare the following reaction system on ice:

reagents	Amount of usage
Template RNAa	*0 ng~1μg
RTase III Primer Flexible All-in-One Mix	4μl
Oligo(dT)*0VN (*0μM)	1μl
or Random hexamers (*0μM)	1μl
or Gene Specific Primers (*0μM)	0.1μl
Nuclease-Free Water	To *0μl

 
a. It is recommended to use high-quality RNA extracted by the kit and freed of genomic DNA contamination as a template.
 
2. Gently pipette to mix and spin;
 
3. Incubate at *5℃ for *5 min;
 
Note: If the target RNA does not contain a Poly(A) structure, it can be pre-incubated at *5℃ for *0 min.
 
4. After the reaction, incubate at *5℃ for 5 min to terminate the reaction;
 
5. Place the obtained cDNA solution on ice for subsequent experiments; or store it immediately at **0℃.
 
Note:
 
1. The subsequent experiment is a cloning experiment. If the RNA comes from eukaryotes, only Oligo (dT) VN is needed. Adding Random hexamers will reduce the yield of full-length cDNA; if the RNA comes from prokaryotes, only Random hexamers or Gene Specific Primers are needed.
 
2. The subsequent experiment is qPCR. Please add Oligo(dT) VN and Random hexamers at the same time to obtain cDNA with uniform reverse transcription efficiency at different positions of mRNA.
 
Notes
 
To prevent RNase contamination, please keep the experimental area clean; wear clean gloves and masks during operation; the consumables such as centrifuge tubes and gun tips used in the experiment must be RNasefree.

Pays:	China
N ° de modèle:	-
Prix FOB:	(Negotiable) Obtenir le dernier prix
Localité:	China
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Groupe de produits :	Molecular Biology