La description
miRNA stem-loop fluorescence quantitative kit
Cat.: MR******5T, MR******0T
Price: $**5 for**0TÂ Â $*8 for *5TÂ Â $*6 for *0T
(negotiable if bulk purhcase)
Storage conditions: transported on dry ice or blue ice, stored at
**0°C away from light, avoid repeated freezing and thawing, valid
for *2 months. If the amount used each time is small, it is
recommended to use in small portions.
| Components |
*5T |
*0T |
|
2× miRNA
SYBR Mastermix
|
1.0ml ×2 |
1.0ml ×4 |
|
Tag Primer |
*0ul |
**0ul |
|
ddH2O |
1.0ml ×2 |
**0μl |
Product Introduction
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miRNA Stem-loop SYBR qPCR Kit uses the chemically modified
hot-start polymerase produced by our company and uses the SYBR
Green I chimeric fluorescence method to perform fluorescent
amplification of miRNA. It has high specificity and can effectively
avoid primer dimer formation and other forms of non-specific
amplification. The reaction buffer has been fully optimized, with
high PCR amplification efficiency, strong fluorescent signal, and
higher reaction sensitivity.
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Product Features
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1. Using chemically modified hot-start polymerase, the
reaction specificity is high, which can effectively avoid
non-specific amplification;
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2. The reaction buffer has been fully optimized, the PCR
amplification efficiency is high, and the reaction sensitivity is
better than many similar products.
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Quality Control
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Using the synthesized U6 as the sample, using this kit,
stem-loop reverse transcription, and qPCR according to the
instructions, the CT value is less than *0.
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