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Pays:
China
N ° de modèle:
-
Prix FOB:
( Negotiable ) Obtenir le dernier prix
Localité:
China
Prix de commande minimale:
-
Commande minimale:
-
Packaging Detail:
-
Heure de livraison:
7-15 days
Capacité de Fournir:
-
Payment Type:
T/T, L/C, D/A, D/P, Western Union, Money Gram, PayPal, Other
Groupe de produits :
Personne àcontacter Ying
CAS：********6
Price: U.S.＄**0.*0—*0g
               Â
$*************5g (negotiable)
Item
number:Â AL***1
Storage
condition:Â room temperature storage, valid for 1
year
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Product
introduction
Agarose is the neutral, uncharged component of
AGAR, consisting of long chains alternately linked by 1, *-linked
β-D-galactose and 1, *-linked 3, *-inner ether-l-galactose. agar is
a polysaccharide, mainly composed of agarose (about *0%) and
agaropectin. Agarose is a neutral substance composed of galactose
and its derivatives, without charge, while agarose is a strong
acidic polysaccharide containing sulfate and carboxylic groups.
Because these groups are charged, they can produce strong
electroosmosis under the action of electric field, and sulfate can
interact with some proteins and affect the electrophoresis speed
and separation effect. Therefore, agar-agar is used as a substrate
for plate electrophoresis to isolate and identify nucleic acids,
such as DNA identification and DNA-restricted endonucrenase
mapping.
Â
Low
melting point agarose is a modified agarose with unique gelling
properties, which hydrates faster than conventional agarose, and
users do not need to boil for too long.
Â
Otherwise, it is easy to lead to thick glue,
which is not conducive to mixing, and easy to damage after gel
(melting point range: ****8 ° C, gel temperature (1.0%) : ****9 °
C). The low melting point agarose gel has better transparency,
better molecular sieve properties, higher electrophoretic band
clarity and high resolution, and is especially suitable for gel
electrophoresis separation and recovery of nucleic acids greater
than ***0bp, as well as preparation of media containing heat
unstable materials.
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Instructions for
use
For
example, 1×TBE solution is prepared with 1% concentration agarose
gel:
1.
The volume of the beaker used to prepare the gel should be **4
times the volume of the gel liquid. Slowly add agarose to the
buffer, stirring while adding to prevent agarose accumulation.
Recording bottle Total body and solution
weight.
2.
Microwave oven on high heat for *0 seconds and adjust the heating
time according to the volume of the prepared solution. The heating
time is related to microwave oven, bottle size and agarose
concentration.
3.
Shake the agarose solution.
4.
Heat it again for *0s on high heat, and shake the agarose
solution.
5.
Put the solution back in the microwave and bring to a boil on high
(about ****5s). Agarose gel may boil violently when moving. Use
caution to avoid burns. Remove from the microwave and let cool at
room temperature for **2 minutes, shaking gently to allow bubbles
to escape.
6.
Return to microwave, heat on high, let boil for about *5s, observe
the dissolved state of agarose, if there are still particles,
repeat this step until all crystals Dissolve it
all.
7.
After the agarose is completely dissolved into liquid, re-weigh the
total weight, calculate the amount of evaporated liquid, fill up
with water to the original weight, shake the liquid
well.
8.
It is recommended that the glue is poured when the liquid is cooled
to ****5℃,
which is conducive to the uniform gel aperture and will not damage
the glue machine. Gently shake the agarose solution before gluing
to release the remaining bubbles in the gel
solution.
9.
Pour glue into the glue making tank, the thickness of the general
glue is **5mm, and try to eliminate the bubbles between the comb
holes or at the bottom after the glue
filling.
*0.
Place at room temperature (****5min) to fully set the
glue.
*1.
If different concentrations and volumes of gel are to be prepared,
please note: Shake the gel solution at least 2 times before
boiling; After boiling, the glue is observed every ****5s check the
degree of dissolution of a glue solution (the interval depends on
the concentration and volume of the glue).
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Matters needing
attention
1.
This product is only used for scientific research by professionals,
shall not be used for clinical diagnosis or treatment, shall not be
used for food or medicine, and shall not be stored in ordinary
homes.
2.
For your safety and health, please wear a lab coat and disposable
gloves.
| Pays: | China |
| N ° de modèle: | - |
| Prix FOB: | ( Negotiable ) Obtenir le dernier prix |
| Localité: | China |
| Prix de commande minimale: | - |
| Commande minimale: | - |
| Packaging Detail: | - |
| Heure de livraison: | 7-15 days |
| Capacité de Fournir: | - |
| Payment Type: | T/T, L/C, D/A, D/P, Western Union, Money Gram, PayPal, Other |
| Groupe de produits : | Molecular Biology |
