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USA
Personne àcontacter Mr. Prime
155 Federal Street, Suite 700, Boston, Massachusetts
Serum, the transparent yellow fluid obtained after coagulation and
centrifugation, contains water, proteins (albumin, fibrinogen, and
globulin, etc.), lipids, carbohydrates, and other substances.
Proteins in serum contain valuable biological information, offering
crucial insights into disease occurrence, progression, prediction,
and diagnosis. Serum proteomics is a comprehensive study of the
types, structures, and functions of proteins in serum. This
research method provides insights into biological processes,
disease mechanisms, and drug targets. As technology advances, serum
proteomics will play an increasingly significant role in biomedical
research.
The primary techniques for serum proteomics analysis include
protein separation and purification techniques, such as gel
electrophoresis, liquid chromatography, etc.; protein
identification techniques, such as mass spectrometry, Edman
degradation, etc.; protein quantification techniques, such as
labeling, label-free methods, etc.; as well as bioinformatics
analysis, such as protein clustering analysis, functional
annotation, and pathway analysis. These methods work together to
advance serum proteomics research.
Analysis Workflow
1. Sample Preparation and Handling
After removing erythrocytes, leukocytes, and platelets, serum is
collected through centrifugation and treated with protease
inhibitors to prevent protein degradation.
2. Protein Extraction and Purification
Proteins are extracted and purified from serum using methods like
salting out, ion exchange chromatography, and affinity
chromatography.
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3. Proteome Separation
Proteins are separated using 1D or 2D gel electrophoresis or liquid
chromatography.
4. Protein Identification and Quantification
Mass spectrometry provides protein molecular weights and peptide
sequences for identification and quantification.
5. Data Processing and Analysis
Software like Mascot, SEQUEST, and MaxQuant is used to process and
analyze mass spectrometry data.
6. Bioinformatics Analysis
Integrating the analysis results with known bioinformatics data
reveals the roles of proteins in biological processes and
diseases.
Applications
1. Drug Target Screening and Drug Development
2. Disease Diagnosis and Biomarker Research
3. Systems Biology Research
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Deliverables
In the technical report, MtoZ Biolabs will provide you a detailed
technical information, including:
1. Experimental Procedures
2. Relevant Mass Spectrometry Parameters
3. Detailed Serum Proteomics Analysis Information
4. Mass Spectrometry Images
5. Raw Data
About Us
MtoZ Biolabs is an integrate contract
research organization (CRO) providing advanced proteomics,
metabolomics, bioinformatics, and biopharmaceutical analysis
services to researchers in biochemistry, biotechnology, and
biopharmaceutical fields. The name of MtoZ represents mass to
charge ratio in mass spectrometry analysis, as most of our services
are provided based on our well-established mass spectrometry
platforms. Our services allow for the rapid and efficient
development of research projects, including protein analysis,
proteomics, and metabolomics programs.
MtoZ Biolabs is specialized in quantitative
multiplexed proteomics and metabolomics applications through the
establishment of state-of-the-art mass spectrometry platforms,
coupled with high-performance liquid chromatography technology. We
are committed to developing efficient, and effective tools for
addressing core bioinformatics problems. With a continuing focus on
quality, MtoZ Biolabs is well equipped to help you with your needs
in proteomics, metabolomics, bioinformatics, and biopharmaceutical
research. Our ultimate aim is to provide more rapid,
high-throughput, and cost-effective analysis, with exceptional data
quality and minimal sample.​
| Pays: | USA |
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