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USA
Personne àcontacter Mr. Prime
155 Federal Street, Suite 700, Boston, Massachusetts
Chemical proteomics, as an important branch of proteomics,
integrates methods used in synthetic chemistry, cell biology, and
mass spectrometry. It aims to design small molecule (SM) probes to
elucidate the mode of action of SMs and the function of protein in
drug discovery. Within the context of natural products, chemical
proteomics can be used to identify the protein binding to or
targets of SMs in living cells. Chemical proteomics consists of two
key steps: (1) probe design and synthesis; (2) target capture and
protein identification. To date, four different types of probes
have been developed to screen for SM targets with various
structures; after targeting, there are also various protein
identification methods suitable for different scenarios. Chemical
proteomics provides a range of novel methods for target
deconvolution, the discovery of drug response biomarkers and
analysis of disease pathway in preclinical target validation. It
has been widely used in pharmacology to identify the target of and
study the mechanism of action of drug.
MtoZ Biolabs utilizes a high-resolution mass spectrometry platform
to develop and validate cutting-edge chemical proteomics
technology, which can be used in numerous fields such as biomarker
research, drug and target discovery, pathway modeling, and action
mechanism research, aiming to provide clients with high-quality and
comprehensive target deconvolution service. We offer a variety of
methods to identify and validate new drug targets to meet diverse
needs.
Designing and synthesizing probes are the initial and critical
steps in the target recognition process of chemical proteomics.
MtoZ Biolabs offers four common types of probes, including: (1)
Immobilized probes: biologically active natural products are
covalently fixed on biocompatible inert resins (such as agarose and
magnetic beads), serving as bait for screening target proteins in
active proteome; (2) Activity-based probes (ABP): considering the
activity of drug molecules, ABP can interact with proteins in
active proteome before enrichment, and can even penetrate cell
membranes to bind with target proteins in living cells, whichÂ
reflectes potential drug-target interactions in cells; (3) Click
chemistry probes: such probes are covalently linked to affinity
tags (biotin-azides, biotin-cyclopropenes) or fluorescent tags
(rhodamine-azides, FITC-azides) through biorthogonal click
reactions with their complements (azides, alkynes, etc.), which can
be used for subsequent enrichment and target identification; (4)
Photoaffinity probes: photoaffinity probes consist of a click
chemistry probe scaffold and a photoaffinity group used to fix the
probe to the target upon binding. The group can produce highly
reactive radicals under specific wavelength light to promote
covalent binding of the active molecule with its target.
For some natural products whose suitable modification sites is
lacking or whose activity is affected by modification, MtoZ Biolabs
provides non-labeled chemical proteomics methods based on drug
affinity responsive target stability (DARTS) and thermal proteome
profiling (TPP). DARTS protect target proteins from proteolytic
enzyme through interactions with SMs. Initially, cell lysates are
divided into aliquots and each aliquot is treated with target
compounds, controls or inactive analogs, followed by limited
proteolysis for the proteins in the cell lysates. Then samples are
separated by SDS-PAGE and stained to identify SM-protein complex
tolerant of proteolysis, and finally, MS is used to identify
targeted proteins.
MtoZ Biolabs possess comprehensive chemical proteomics solutions
composed of experimental design, sample preparation, probe
synthesis, target identification, and data analysis, offering a
one-stop service to meet your needs in drug development and
biomarker research, drug and target discovery, pathway modeling,
and drug action studies. With our comprehensive solutions, you can
save time and costs in search of drug candidates, and can optimize
drug development and discovery with high success rate. Welcome your
consult for more detailed information!
Service at MtoZ Biolabs
1. Experimental Procedures
2. Relevant Mass Spectrometry Parameters
3. Detailed Chemical Proteomics Information
4. Mass Spectrometry Images
5. Raw Data
About Us
MtoZ Biolabs is an integrate contract
research organization (CRO) providing advanced proteomics,
metabolomics, bioinformatics, and biopharmaceutical analysis
services to researchers in biochemistry, biotechnology, and
biopharmaceutical fields. The name of MtoZ represents mass to
charge ratio in mass spectrometry analysis, as most of our services
are provided based on our well-established mass spectrometry
platforms. Our services allow for the rapid and efficient
development of research projects, including protein analysis,
proteomics, and metabolomics programs.
MtoZ Biolabs is specialized in quantitative
multiplexed proteomics and metabolomics applications through the
establishment of state-of-the-art mass spectrometry platforms,
coupled with high-performance liquid chromatography technology. We
are committed to developing efficient, and effective tools for
addressing core bioinformatics problems. With a continuing focus on
quality, MtoZ Biolabs is well equipped to help you with your needs
in proteomics, metabolomics, bioinformatics, and biopharmaceutical
research. Our ultimate aim is to provide more rapid,
high-throughput, and cost-effective analysis, with exceptional data
quality and minimal sample.​
| Pays: | USA |
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| Prix FOB: | Obtenir le dernier prix |
| Localité: | - |
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