Description
Name:2*Taq PCR Master (without dye)
Cat. No.: T***4
Product number: T***4
Storage conditions: **0℃
Product description
The concentration of Taq-ASPCRMix is ​​2×. It is easy and quick
to use and can reduce contamination during PCR operation. When
using, just take an appropriate amount of 2×Taq-AS PCR Mix, add
template and primers, and add ddH2O to make up the volume. Make the
reaction system concentration 1×, and then you can carry out PCR
reaction. The 3\' end of the PCR product has a prominent A base and
can be directly used for T/A cloning after purification.
The TaqDNA polymerase in this Mix is ​​a screened Taq-AS
(Advanced Strong) DNA Polymerase mutant, which can efficiently
amplify DNA fragments ≤5kb and has good inhibitor tolerance. Its
amplification speed is about 3 times that of ordinary Taq DNA
polymerase, so it can greatly reduce the time required for the PCR
extension process, thereby shortening the entire PCR reaction.
Different from the fast Taq polymerase based on the fusion protein
principle, the use of Taq-AS is closer to WT-Taq, and it is not
easy to have electrophoretic band diffusion, dragging or fragment
size changes.
Quality control
Nuclease activity detection
*5μl 2×Taq-AS PCR Mix and **0ng supercoiled plasmid DNA were
prepared into a *0μl reaction system. After incubation at *7℃ for
4h, less than *0% of the plasmid DNA was converted into a nicked or
linear state by agarose gel electrophoresis.
Nonspecific nuclease activity detection
*5μl 2×Taq-AS PCR Mix and *5ng double-stranded DNA fragment were
prepared into a *0μl reaction system. After incubation at *7℃ for
*6h, agarose gel electrophoresis was used to detect that there was
no change in the double-stranded DNA substrate.
