Prix FOB
Obtenir le dernier prix( Negotiable )
|500 Gram Minimum Order
Pays:
China
N ° de modèle:
DS-TRIS-04
Prix FOB:
( Negotiable ) Obtenir le dernier prix
Localité:
Ezhou city,Hubei province,china
Prix de commande minimale:
-
Commande minimale:
500 Gram
Packaging Detail:
500g / bottle, 25kg / barrel
Heure de livraison:
5 days
Capacité de Fournir:
100 Ton per Month
Payment Type:
T/T, L/C, PayPal, Other
Groupe de produits :
Personne àcontacter jing zhao
Business Address:C8-2, Optics Valley United Technology City, Huarong District, Ezhou, Hubei
High-quality Tris
buffer is an important part of electrophoresis. It allows current
to pass through the sample while resisting changes in the pH value
of the entire solution. The choice of buffer depends on the
isoelectric point of the sample being analyzed. In DNA
electrophoresis, the commonly used buffers are Tris-Acetate-EDTA
and Tris-Borate-EDTA. In protein electrophoresis, SDS (sodium
dodecyl sulfate) is usually used. These buffers help separate
samples into readable gels. Depending on the sample, other buffers
can help improve results or expand readings at specific molecular
weights.
Recently, I have
obtained some knowledge about electrophoresis buffer through
professional literature, and I want to share and discuss with you
here.
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The role
of Tris-acetate-EDTA and Tris-borate-EDTA in agarose gel
electrophoresis:
Tris is a strong
base and boric acid is an acid. The combination of the two can keep
the pH in the neutral range of 8 to 8.5. Under such alkaline
conditions, DNA is protected and can be separated properly. EDTA
plays an important role in this combination. EDTA is a chelating
agent. It chelates the Mg2+ ions required by the DNAse enzyme as a
cofactor. DNAse is an enzyme that cuts DNA into small fragments.
Therefore, by adding EDTA, we can protect our DNA from enzymatic
activity. In addition, the buffer will neutralize the charge of the
water molecules. Under the action of electric current, water
molecules decompose into H + and OH-. H + ions can react with the
PO*- of DNA. Therefore, the negative charge of the buffer will
neutralize the charge in the water and protect the DNA.
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Although
Tris-Acetate-EDTA and Tris-Borate-EDTA have the same role in
electrophoresis, they have their own advantages and disadvantages.
Tris-acetate-EDT buffer can interfere with enzymatic reactions and
protect DNA, while Tris-borate-EDTA buffer cannot.
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In summary, the
use of Tris-acetate-EDTA and Tris-borate-EDTA derived from Tris
buffer in electrophoresis is very important, and this is one of the
reasons why Tris buffer is in short supply in todays market. One.
Last time now, it was very difficult to find a manufacturer
specializing in the production of Tris, but here I would like to
recommend our company, Hubei Xindesheng Materials Co.,
Ltd.
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Hubei New Desheng
Material Co., Ltd. specializes in the production of Tris, Hepes,
Mops and other buffer products. Up to now, the company has been
established for decades and has very rich experience in the
development and production of biological buffers. We can provide a
large number of Tris and other types of biological buffer products,
and our company can provide product customization and professional
after-sales service for customers, and provide professional product
sample testing services and packaging customization services for
special customers. In any case, Desheng Company must be your best
choice for purchasing biological buffers.
| Pays: | China |
| N ° de modèle: | DS-TRIS-04 |
| Prix FOB: | ( Negotiable ) Obtenir le dernier prix |
| Localité: | Ezhou city,Hubei province,china |
| Prix de commande minimale: | - |
| Commande minimale: | 500 Gram |
| Packaging Detail: | 500g / bottle, 25kg / barrel |
| Heure de livraison: | 5 days |
| Capacité de Fournir: | 100 Ton per Month |
| Payment Type: | T/T, L/C, PayPal, Other |
| Groupe de produits : | Tris(Hydroxymethyl)Aminomethane |
