Tris buffer solution CAS No. 77-86-1 configuration and use

La description

Tris is one of the common biological buffers, which is very widely used and plays a role in many fields. To configure high purity Tris, the process is still relatively complex, requiring nitromethane, hydrochloric acid, hydrochloric acid, methanol and activated carbon and other materials,
The specific preparation procedure is as follows:
The configuration of Tris is generally carried out with hydrochloric acid or EDTA. Of course, there are also tris buffers directly configured with deionized water and tris powder, which only need to be deployed to the required concentration. However, the configuration with hydrochloric acid or EDTA requires more steps.
How to configure Tris-HCl buffer solution:
1. Prepare measured trihydroxymethylaminomethane (Tris) and hydrochloric acid (HCl).
2. The required dosage of Tris and HCl was calculated according to the required pH value, and the ratio of Tris-HCl was 1:1.
3. Mix Tris with appropriate amount of deionized water, gradually add HCl to adjust pH value while stirring, and finally adjust the volume to the required volume.
4. Adjust the pH value to the desired value with 0.1mol/L hydrochloric acid or sodium hydroxide solution.
5. Filter the liquid to remove any undissolved impurities. Tris buffer can be used in:
1. Application of biochemical experiments: TRIS is used in the preparation of buffer solution in biochemical experiments, such as TAE, TBE and other extended buffer solution are prepared by TRIS.
2, cosmetics acid-base regulation: can neutralize the PH value of raw materials in cosmetics, make cosmetics suitable for skin pH.
3. Pharmaceutical additive: Tris can be used to make ammonium bradylate injection, which is a drug used to regulate acid-base balance and is generally used to treat acute respiratory and metabolic acidemia.
4, chemical coatings: Tris added to the paint to adjust the PH value, prevent the paint from oxidation and discoloration, but also increase the viscosity of the paint.
6, environmental activated carbon: Tris used in the air purification of activated carbon, can improve the adsorption efficiency, extend the service life of activated carbon.
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