La description

Cat. No. CD**1

Ordering Information

 

Storage: at **0oC for six months

Description

Trans*-T1 Phage Resistant Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over **9cfu/μg DNA (tested by pUC*9 plasmid DNA).

Genotype

F-φ*0(lacZ)ΔM*5ΔlacX*4hsdR(rk -, mk +)ΔrecA***8endA1tonA

Features

• High transformation efficiency: >**9 cfu/μg ( pUC*9 DNA).

• Fast-growing, doubling time about *0 minutes and colonies are visible in 8~9 hours.

• Resistance to T1 and T5 phage.

• Blue/white selection.

Procedures

• Thaw a vial of **0 μl Trans*-T1 Phage Resistant Chemically Competent Cell on ice, aliquot *0 μl of the cells into a prechilled 1.5 ml tube, add target DNA into the tube and mix gently. Incubate the cells on ice for *0 minutes.

• Heat-shock the cells for *0 seconds at *2oC, and then quickly remove the tube from the *2oC water bath and place them on ice for 2 minutes. Do not shake the tube during this procedure.

• Add **0 μl of sterile SOC medium or LB medium (no antibiotic) into the tube, mix well and shake at *7oC for 1 hour at **0 rpm to resuscitate cells.

• According to the experiment requirement (plasmid, transformation of recombinant ligation product), add different volumes of transformed cells into corresponding antibiotic-containing SOB or LB medium. Spread the transformed cells on selective plate. Invert the plate and incubate at *7oC overnight.

 

Notes

• High efficiency transformation can be achieved by transforming cells immediately following thawing.

• Avoid repeated thawing.

• Avoid pipetting cells.

• Gentle handling is required for the entire procedure.

 

CITATIONS

Du F, et al. ***3. RNA pathogen detection with one-step reverse transcription PCR and strand-displacement based signal amplification. **8(5):*****8.Analyst. IF=3.**9. PMID: *******5.

Zhang X, et al. ***3. The Different Potential of Sponge Bacterial Symbionts in N2 Release Indicated by the Phylogenetic Diversity and Abundance Analyses of Denitrification Genes, nirK and nosZ. 8(6):e****2. PLoS One. IF=3.*3. PMID: *******0.

Jiang Q, et al. ***3. A Scallop Nitric Oxide Synthase (NOS) with Structure Similar to Neuronal NOS and Its Involvement in the Immune Defense. 8(7):e****8.  PLoS One. IF=3.*3. PMID: *******8.

Shen F, Yao M. ***3. Are We Biologically Safe with Snow Precipitation? A Case Study in Beijing. 8(6):e****9. PLoS One. IF=3.*3. PMID: *******7.

Sun X Q, et al. ***3. Glutathione S-Transferase of Brown Planthoppers (Nilaparvata lugens) Is Essential for Their Adaptation to Gramine-Containing Host Plants. 8(5):e****6. PLoS One. IF=3.*3. PMID: *******0.

Han M, et al. ***3. The ammonia oxidizing and denitrifying prokaryotes associated with sponges from different sea areas. *6(2):*****6. Microb Ecol. IF=3.**7. PMID: *******7.

Wei X, et al. ***3. A four-domain Kunitz-type proteinase inhibitor from Solen grandis is implicated in immune response. *3(6):******4. Fish Shellfish Immunol. IF=2.**4. PMID: *******4.

Li X, et al. ***3. Polymorphisms of anti-lipopolysaccharide factors in the swimming crab Portunus trituberculatus and their association with resistance/susceptibility to Vibrio alginolyticus. *4(6):*****8. Fish Shellfish Immunol. IF=2.**4. PMID: *******7.

 

Zhou Z, et al. ***1. A dopa decarboxylase modulating the immune response of scallop Chlamys farreri. 6(4):e****6. PLoS One. IF=3.*3. PMID: *******0.

Zhou K, et al. ***1. Phylogenetically diverse cultivable fungal community and polyketide synthase (PKS), non-ribosomal peptide synthase (NRPS) genes associated with the South China Sea sponges. *2(3):*****4. Microb Ecol. IF=3.**7. PMID: *******3.

Zhou Z, et al. ***1. A monoamine oxidase from scallop Chlamys farreri serving as an immunomodulator in response against bacterial challenge. *5(7):******7. Dev Comp Immunol. IF=3.**8. PMID: *******4.

Zhou Z, et al. ***1. Scallop phenylalanine hydroxylase implicates in immune response and can be induced by human TNF-α. *1(6):*****3. Fish Shellfish Immunol. IF=2.**4. PMID: *******0.

Yu H Y, et al. ***1. Development of polymorphic microsatellite markers for Incarvillea sinensis (Bignoniaceae). *8(8): e***-e**5. Am J Bot. IF=2.**6. PMID: *******6.  

Tan Y, Ji G. ***0. Bacterial community structure and dominant bacteria in activated sludge from a *0 degrees C ultrasound-enhanced anaerobic reactor for treating carbazole-containing wastewater. **1(1):*****0.Bioresour Technol. IF=4.*5. PMID: *******5.