La description
PRINCIPLE
The DairyPal
BST is
competitive test involving three receptors in one single
operation, the β-lactams, sulfonamides and
tetracyclines antibiotics
in the raw milk sample compete for the antibody with the
antigen coated on the membrane of the test dipstick. Then
after a
color reaction, when the sample is free of antibiotics, a
colour development occurs at the specific capture lines,
indicating the absence
of the concerned analytes in the milk sample. On the contrary,
the presence of antibiotics in the sample will not cause
the coloured
signal to appear at the specific capture lines.
APPLICATIONS
The DairyPal
BST is a
rapid test that detect simultaneously the presence of either
β-lactam, sulfonamide and tetracycline
molecules in a
raw cow / goat milk sample.
TEST PROCEDURE
1. Read the instruction attentively, choose a clean
and dry place to perform the test, wash and dry your hands before
starting.
2.Take
the kit out of the fridge and wait until the temperature of the
reagents reaches the ambient temperature.
3.Calculate
how many milk sample will be tested and mark the sample tubes to
identify. The milk samples should be fully
liquid without
any clots or deposition, the ideal temperature of milk sample is
between 4 to *0℃.
4.Take
the tubes required from the kit package, take out as many
micro-wells and dipsticks as the milk samples to be tested,
and make
proper marks to match the milk sample.
ï¬To
open a dipsticks tube, press the safety ring down, take off the
ring and get the tube cap off with your thumb.
ï¬Recover
the cap tightly after taking out the required micro-wells and
dipsticks.
ï¬Empty
one test tube then open another one.
ï¬Keep the unused
micro-well caps tightly sealed. Do
NOT tear off the
strip of 8 microwell caps.
ï¬Use
the exposed dipsticks in 1 hour.
5.Transfer
**0ul of the milk samples into the microwells, inject and reject
the milk sample for 5 times consecutively
to homogenize
the lyophilized reagent and milk sample. Start the timer.
ï¬Dip
the end of the plastic pipette(or MilkPal Mini pipette tips) into
the milk and slowly releases the sucker. When
the sucker
is back into its initial position, transfer the **0 µl of milk
into the reagents, by slowly pushing the sucker down to
the bottom.
6.Start the timer
to incubate for 3 minutes at room
temperature (*5℃);
ï¬During
the incubation, The receptors will detect the presence of the
antibiotics in the milk sample. The reaction will take
3 minutes.
7.Dip the
dipsticks into the micro-wells with the
"Sample
Pad" down side to
the bottom.
8.Start the timer
to incubate for another 6 minutes again
at room temperature.
9.Then
take out the dipsticks and place them laterally in a dry clean
place, peel off the sample pad, interpret the result.
ï¬Use
a clean nipper(or new pipette tip) to scrape gently and slowly
the sample pad.
ï¬The
peeling off of the sample pad will stop the ongoing reaction of
the receptors.
ï¬File
the used sample pad peeled off dipsticks if necessary.
RESULTS
INTERPRETATION
The dipsticks
are embodied with 4 lines, Control
line, Tetracylcines
Line, Sulfonamides
Line and β-lactams
Line, and
which are briefly
expressed as C, T, S and B. The test
results will be determined by the color presence of the lines.
The following diagram
describes the result identification.
Method A
(Visual
Interpretation)
ï¬NEGATIVE:
C line, T Line, S Line
and B Line are all
red, Color of T line, S line, B Line are all
stronger or similar than the C line,which
means that, the milk sample contains fewer antibiotics than value
given in the table.
ï¬β-lactams POSITIVE:
C Line is red, B
Line is weaker than C line, which
means that the milk sample contains the
beta-lactams more
than the value in the table.
ï¬Sulfonamides
POSITIVE: C Line is
red, S Line is weaker
than C line, which
means that the milk sample contains
the sulfonamides
more than the value in the table.
ï¬Tetracyclines
POSITIVE: C Line is
red, T Line is weaker
than C line, which
means that the milk sample contains
the tetracyclines
more than the value in the table.
ï¬Tetracyclines, Sulfonamides and β-lactams
POSITIVE: C Line is red;
neither T Line, S Line
or B Line is weaker
than C Line;
which means
that, the milk sample contains the tetracyclines, sulfonamides
and beta-lactams all more than the value in
the table.
Remarks: Control line is used as a quality indicator,
which will always appear regardless of the T/S/B line. If Control
line does NOT appear,
it indicates that the result is invalid. Users please
check the kit insert again and re-do the assay with new test
dipsticks.
SPECIFICITY
The dipstick
shows negative with the melamine, chloramphenicol,
streptomycin, lincomycin spiking at the level
of **0μg/L.
STORAGE
**8℃ in cool dark place, do not freeze. The kit
will be valid in *2 months. The lot number and expired date are
printed on the package.
PRECAUTIONS
ï¬Do the assay
following the instruction, do NOT touch the
membrane of the dipstick.
ï¬Seal
the bottle after taking out required dipsticks.
ï¬The dipstick
is used for only once; Do NOT use it
repeatedly.
ï¬The
positive result should be further confirmed.
| Pays: |
China |
| N ° de modèle: |
SC060
|
| Prix FOB: |
75 ~ 85 / Box ( Negotiable ) (Negotiable)
Obtenir le dernier prix
|
| Localité: |
China |
| Prix de commande minimale: |
75 per Box |
| Commande minimale: |
1 Box |
| Packaging Detail: |
carton |
| Heure de livraison: |
3 days for ready goods |
| Capacité de Fournir: |
9000 Piece per Month |
| Payment Type: |
T/T, L/C, D/A, D/P, Western Union, Money Gram, PayPal |
| Groupe de produits : |
3in1
|
