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Business Address:C8-2, Optics Valley United Technology City, Huarong District, Ezhou, Hubei
SUMO Protease, also known as Ulp, is a cysteine protease with high
activity. It can recognize the tertiary structure of SUMO protein,
rather than the amino acid sequence, so it can efficiently and
specifically cut sumo protein from the recombinant fusion protein.
SUMO Protease can maintain high activity in a wide range of
reaction environment system, such as temperature (***0 ℃), pH
(5.**9.5), and so on. SUMO Protease has his label, which is
convenient to remove the protease by affinity chromatography after
cutting the fusion protein.
Storage conditions:
SUMO Protease can be stored for 2 years at - *0 ℃ , and can be
stored at - *0 ℃ after the first use to avoid repeated freezing
and thawing. *0 × SUMO Protein Buffer can be stored at - *0
℃.
Definition of enzyme activity:
In 1 × SUMO Protein Buffer (*0 mM Tris-HCl, 1 mM DTT, pH 8.0), the
amount of enzyme needed for reaction at *0 ℃ for 1 hour and
cutting 2 μg substrate with more than *5% was defined as an active
unit.
Instructions for use:
1. *0×SUMO Protease Buffer :**0mM Tris-HCl, *0mM DTT, pH 8.0。
2. The ratio of SUMO Protease to the target protein to be digested:
1:**0.
3. Enzyme cutting system:
Fusion protein ***0 μg
*0×SUMO Protease Buffer *0μL
SUMO Protease 2 μL
ddH2O constant volume to ***0 μ L
4. Enzyme cutting conditions: 4 ℃ is recommended for overnight
enzyme cutting. Users can explore the target protein according to
their own research. The following image of enzyme cutting analysis
can be used for reference.
5. After enzyme cutting, a small number of samples can be taken for
SDS-PAGE analysis. To remove SUMO Protease in the system after
enzyme cutting, the resin affinity chromatography can be purified
by His tag.
| Pays: | China |
| N ° de modèle: | - |
| Prix FOB: | Obtenir le dernier prix |
| Localité: | - |
| Prix de commande minimale: | - |
| Commande minimale: | - |
| Packaging Detail: | - |
| Heure de livraison: | - |
| Capacité de Fournir: | - |
| Payment Type: | - |
| Groupe de produits : | In Vitro Diagnostic Kit Raw Materials |
