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Personne àcontacter Louis
Shanghai, Shanghai
Recombinant insulin-like growth
factor
CAS: *********6
Product name: Recombinant human insulin-like growth factor*1
(IGF*1)
-Product
Introduction
Insulin-like growth factor*1 (IGF*1), also known as SomatamedinC,
belongs to the insulin-like growth factor family and has structural
homology with proinsulin. IGF*1 is a kind of polypeptide that
promotes mitosis. It stimulates the proliferation and survival of
muscle, bone, cartilage and other cells in vitro. IGF*1 can combine
with IGF*1R, IGF*2R and insulin receptor, awaken multiple signal
pathways, and participate in cell proliferation, differentiation,
survival, growth, apoptosis and regeneration. Recombinant human
insulin-like growth factor (IGF*1), composed of *0 amino acids, is
a bioactive protein for cell culture. This strain was expressed by
recombinant yeast, purified by multi-step separation, and then 0.*2
μ M The lyophilized powder is made by freeze-drying after
filtration and bacteria removal.
-Product
advantages
No animal origin: no animal virus, no pathogenic substances, no
foreign factor pollution, high safety and security.
Quality stability: This product is a lyophilized powder containing
biological active protective agent. It can be produced in batches
with small difference between batches.
The production scale is more than ***0L.
Compliance: The production equipment and production environment
comply with the requirements of relevant laws and regulations, and
comply with the guiding principles of GMP.
-Product
characteristics
Speciesman
Source Pichia pastoris
White or almost white lyophilized powder
Purity ≥ *5%
(SDS-PAGE)
Specific activity ≥ 2.2
× **5 IU/mg pro.
Molecular weight 7.7 KD ± 0.8kDa
Endotoxin<0.2 EU/µ g
Isoelectric point 8.4 - 8.6
The dissolution point has good solubility in water, buffer solution
and culture medium
Biological activity: The biological activity of IGF*1 was
determined by its stimulating effect on human breast cancer cell
line MCF*7.
-Product
use
Widely used in cell culture, it can enhance cell proliferation,
cell growth and cell differentiation.
-Usage
It is recommended that the powder be precipitated at the bottom of
the tube by centrifugation for a short time before capping, and
then dissolved with sterilized deionized water to prepare **0 μ
G/ml storage solution can be stored stably for one week at 2
℃~8 ℃. Dilute to a lower concentration
(not less than *0 μ G/ml),
0.1% tissue culture grade BSA or HSA needs to be added. If the
storage time needs to be extended, it is recommended to freeze at -
*0 ℃ or - *0
℃, and it is not
recommended to freeze and thaw repeatedly.
The best concentration of this product should be determined by
experiment according to each specific application.
-Transport and storage
stability
Transportation stability: ice bag transportation, stable
activity.
Storage stability: stable to *4 months at - *5 ℃ and below, and to 4 weeks at 2~8
℃. Compounded as
**0μ G/ml storage solution
can be stored stably for one week at 2~8 ℃.
-Notes and
Disclaimer
This product should not be stored for a long time in high
temperature environment.
Avoid contamination during the operation of this product.
This product is for research use only, and shall not be used for
diagnosis or treatment of animals or humans.
Product No：KK-T**5
