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Localité:
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Prix de commande minimale:
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Commande minimale:
1 Milliliter
Packaging Detail:
bottle or tube
Delivery Time:
within 7days based on stock available
Supplying Ability:
10 Liter per Month
Payment Type:
T/T
Personne àcontacter Louis
Shanghai, Shanghai
Recombinant
Enterokinase
High specificity and high
purity
1. Product
introduction
Enterokinase is a high-purity
recombinant bovine enterokinase. The enzyme is purified by HPLC
with high purity, high specificity and no other proteases.
Enterokinase (EC 3.4.*1.9) is a specific protease that can cleave
the carboxy-terminal site of lysine containing four aspartic acids
in the front: Aspartic acid-Aspartic acid-Aspartic acid-Aspartic
acid Aspartic acid-lysine. Enterokinase can remove the fusion
protein located at the N-terminus of the protein to remove unwanted
fusion tags.
2. Product
advantages
high purity
High specificity
1) A specific protease cleaves the
carboxy-terminal site of lysine containing four aspartic acids in
front: aspartic acid-aspartic acid-aspartic acid-aspartic
acid-lysine (DDDDK)
2) No other proteases, no non-specific
cutting
Figure 1: SDS-PAGE detection result, a
single main band of purified enterokinase
Figure 2: SDS-PAGE analysis of 1U of EK
on *0ug substrate 0min, *0min, *0min, *0min, *0min, *0min,
*0min
Figure 3: SDS-PAGE analysis of 1U, 2U,
5U, *0U EK on *0ug substrate for 4h and 1U EK on *0ug substrate for
*6h
Enzyme digestion conditions:
According to the definition of enzyme
activity, an example of recombinant EK digestion conditions: In
*5mM Tris-HCl 8.0 system:
Fusion protein concentration 0.**1mg/ml
(total protein *****0µg)
Reconstituted EK dosage **2U
Temperature *5℃
Overnight digestion, or complete
digestion requires *2h**6h.
Common factors affecting enterokinase
activity
At >**0mM imidazole, or
>**0mMNaCl, or >5% glycerol, the digestion effect will be
affected. You can refer to the following recommended methods for
digestion:
1) In order to obtain an ideal digestion
result, please dialyze the sample into *5mM Tris-HCl 8.0 buffer,
and then perform digestion.
2) If it is inconvenient for dialysis,
you can dilute the sample. The imidazole content is below **0mM,
the NaCl concentration is below *0mM, and the glycerol
concentration is below 5% for digestion, and the ratio of enzyme
dosage to protein remains unchanged (ie 1U digestion *0μg protein)
.
3) If the sample solution contains one
or more of the above components and it is inconvenient to remove,
at this time, increase the enzyme amount or extend the digestion
time to achieve better digestion results.
3. Product
use
Used to remove the fusion protein
located at the N-terminus of the protein to remove unwanted fusion
tags
4. Product
features
Source Recombinant Escherichia
coli
Theoretical MW: *5,**0DA
Activity 1U is defined as storing *0ug
at *5℃,
within *2h~*6h
The amount of enzyme required to cleave
*5% of the fusion protein in *5mM Tris-HCl 8.0 buffer.
Storage Store at **0°C.
Stability It can be transported at room
temperature and can be stored stably for one week at
*5°C.
The enzyme solution can be repeatedly
frozen and thawed several times without affecting the enzyme
activity.
5. Product
Information
Product name: Recombinant enterokinase
Product number: KK-T**8
Specific activity: ≥5u/μl
