Description
Peptides are normally supplied as a fluffy, freeze-dried material
in serum vials. Store peptides in a freezer after they have been
received. In order to reconstitute the peptide, distilled water or
a buffer solution should be utilized.
Some peptides have low solubility in water and must be dissolved in
other solvents such as 6 to *0% acetic acid for a positively
charged peptide
Use the minimal amount of these non-aqueous solvents and add water
or buffer to make up the desired volume. After peptides are
reconstituted, they should be used as soon as possible to avoid
degradation in solution.
The long-term storage of peptide samples presents a somewhat
different problem. Lyophilized peptides generally have excellent
stabilities (in most cases, lyophilizates can be stored for years
at **0 C or lower temperatures with little or no degradation),
but, in solution, they generally have much more limited
stabilities. Since peptides are susceptible to degradation by
proteases of bacterial or microbial origin, the first rule is to
prepare sterile solutions, either by reconstitution in sterile,
distilled water, or by sterile filtration after reconstitution
