Description

The Immunoglobulin M Test Kit (Immunoturbidimetric Assay), IGM is a quantitative turbidimetric test for the measurement of IgM in human serum or plasma.
Anti-human IgM antibodies when mixed with samples containing IgM, form insoluble complexes. These complexes cause an absorbance change, dependent upon the IgM concentration of the patient sample, that can be quantified by comparison from a calibrator of know IgM concentration.
 For in vitro diagnostic use only.【Applied analyzer】
Spectrophotometer or biochemical analyzer with **0nm wavelength.
【Sample】
Fresh serum or plasma. Do not use highly hemolized or lipemic samples. If not determine in time, store at **0 ℃. Avoid repeat freezing and melting.
【Procedure】
1. Assay method：two point，wavelength **0nm
2.   Application on chemistry analyzer:
Sample Reagent（R1/R2） Temperature Reaction time（T1/T2）
3μL **0μL/*0μL *7℃ **0s./ **0s.

Unit Reaction Direction  Standard curve simulation formula
g/L Positive Multi point calibration, non-linearity calibration mode
3.Determination: Add 3uL samples into **0μL R1,mix thoroughly and incubate the cuvette  at *7℃for **0 seconds. Read the absorbance A1 against the blank.. Then mix *0μL R2 thoroughly and incubate the cuvette for **0 seconds, read the absorbance A2. The absorbance of complex is △A（A*-A1）.
Note: different chemistry analyzer has own specifications, but should be subject to the basic specifications herein above, and  the kit could be used after setting on the chemistry