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Personne àcontacter jing zhao
Business Address:C8-2, Optics Valley United Technology City, Huarong District, Ezhou, Hubei
Name: trimethylolminomethane acetate
English Name: tris (hydroxymethyl) aminomethane acetate salt
CAS No.: ********8
Molecular formula: C4H*1NO3•C2H4O2
Molecular weight: **1.*9
Storage conditions: Store at RT
Application: TAE electrophoresis buffer is the most commonly used
buffer for DNA agarose gel electrophoresis, and is also used in non
denatured RNA agarose gel electrophoresis. Double stranded DNA
tends to move faster in TAE than in other buffers, but it will also
be unable to swim due to the depletion of buffer ions during
long-term electrophoresis. Buffer circulation or replacement during
long-term electrophoresis can compensate for the low buffering
capacity of the chemical book. Dilute the concentrated TAE buffer
to obtain 1mmtae buffer, containing *0mMTris acetate and 1mMEDTA,
with a pH of 8.3. 1mMTAE buffer can be used in agarose gel as well
as electrophoretic buffer. In order to achieve the maximum
resolution, it is recommended that the applied voltage be less than
5V/cm (unit distance between electrodes). TAE electrophoresis
buffer is the most commonly used buffer for DNA agarose gel
electrophoresis, and is also used in non denatured RNA agarose gel
electrophoresis. Double stranded DNA tends to move faster in Tae
than in other buffers, but it will also be unable to swim due to
the depletion of buffer ions during long-term electrophoresis. The
low buffering capacity of chemical book can be compensated by
buffer circulation or buffer replacement during long-term
electrophoresis. Dilute the concentrated TAE buffer to obtain
1mMTAE buffer, containing *0mMTris acetate and 1mMEDTA, with a pH
of 8.3. 1mMTAE buffer can be used in agarose gel as well as
electrophoretic buffer. In order to achieve the maximum resolution,
it is recommended that the applied voltage be less than 5V/cm (unit
distance between electrodes). In the detection of luciferase ATP
with firefly, the product is the most sensitive buffer; glutamate
binding detection.
| Pays: | China |
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| Groupe de produits : | Biological buffer |
