Recombinant nuclease

La description

INDUMY nuclease is a recombinant endonuclease expressed in E.coli and purified to homogeneity. This enzyme attacks and degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) and is effective over a wide range of conditions. The enzyme completely digests nucleic acids to 5'-monophosphate terminated oligonucleotides 2–5 bases in length which is below the hybridization limit. NDUMY nuclease preparations possess exceptionally high specific activities and are supplied free from measurable protease activities and viral contaminants.

This enzyme is ideal for a wide variety of applications where complete digestion of nucleic acids is desirable.

• Viscosity reduction in protein extracts

• Sample preparation for 2D gel electrophoresis

• Removal of nucleic acid contaminants from recombinant protein preparations

This enzyme is designed with (His)6 tag and so it can be easily removed during the downstream procedures. It is functional between pH 6 and *0 and from ***2°C and requires **2 mM Mg2+ for activation. The enzyme is also active in the presence of ionic and non-ionic detergents, reducing agents, The product is supplied as a 0.2 μm filtered solution in *0% glycerol.

Unit definition

One unit of INDUMY™ Nuclease is defined as the amount of enzyme that causes a ΔA**0 of 1.0 in

*0 min, which corresponds to complete digestion of *7 μg of DNA. Standard reaction conditions

are 1 mg/ml sonicated DNA substrate in *0 mM Tris-HCl pH 8.0, 0.1 mg/ml BSA, 1 mM MgCl2,

incubated at *7°C; measured as perchloric acid-soluble digestion product.

Storage

INDUMYâ„¢ Nuclease is supplied in *0% glycerol containing *0 mM Tris-HCl pH 8.0,*0 mM NaCl

and 2 mM MgCl2. The enzyme preparation is stable for 2 years when stored at –*0°C.

DO NOT store at –*0°C