La description
intended use
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This immunoassay kit allows for the in vitro quantitative
determination of Human
Sclerostin (SOST) concentrations in serum, Plasma, Urine,
tissue homogenates and Cell culture supernates and Other
biological fluids.
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sample preparation
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1. DL Sci&Tech Development Co.Ltd is only responsible for the
kit itself, but not for the samples consumed during the assay.
The user should calculate the possible amount of the samples used
in the whole test. Please reserve sufficient samples in advance.
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2. Please predict the concentration before assaying. If values
for these are not within the range of the standard curve, users
must determine the optimal sample dilutions for their particular
experiments.
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3.If the samples are not indicated in the manual, a preliminary
experiment to determine the validity of the kit is necessary.
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4. Tissue or cell extraction samples prepared by chemical lysis
buffer may cause unexpected ELISA results due to the impacts from
certain chemicals.
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5. Due to the possibility of mismatching between antigen from
other origin and antibody used in our kits (e.g., antibody
targets conformational epitope rather than linear epitope), some
native or recombinant proteins from other manufacturers may not
be recognized by our products.
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6. Influenced by the factors including cell viability, cell
number or sampling time, samples from cell culture supernatant
may not be detected by the kit.
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7. Fresh samples without long time storage is recommended for the
test. Otherwise, protein degradation and denaturalization may
occur in those samples and finally lead to wrong results.
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calculation of the result
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Average the duplicate readings for each standard, control, and
samples and subtract the average zero standard optical density.
Create a standard curve on log-log graph paper, with Human Sclerostin (SOST)
concentration on the y-axis and absorbance on the x-axis. Draw
the best fit straight line through the standard points and it can
be determined by regression analysis. Using some plot software,
for instance, curve expert 1.*0, is also recommended. If samples
have been diluted, the concentration read from the standard curve
must be multiplied by the dilution factor.
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Note
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The Stop Solution suggested for use with this kit is an acid
solution.Wear eye,hand,face and clothing protection when using
this material.
| Pays: |
China |
| N ° de modèle: |
-
|
| Prix FOB: |
600 ~ 700 / ( Negotiable ) (Negotiable)
Obtenir le dernier prix
|
| Localité: |
- |
| Prix de commande minimale: |
600 |
| Commande minimale: |
1 |
| Packaging Detail: |
ice bag |
| Heure de livraison: |
5-7 working days |
| Capacité de Fournir: |
- |
| Payment Type: |
T/T |
| Groupe de produits : |
- |
